Sentence examples for spotting procedure from inspiring English sources
In addition, since proteins are cross-linked to the immobilized cDNA just prior to the assay, they are not required for the spotting procedure.
The spotting procedure was conducted under vapor-saturated conditions maintaining the robot atmosphere slightly over the dew point to prevent evaporation of the drops with temperature controlled via a cooling block.
The spotting procedure was done in cooperation with the University of Marburg Genomic Solutions OmniGridd).
The spotting procedure was carried out using split pins (type SMP4, TeleChem, Sunnyvale, CA) in a hood set at 55% humidity.
Due to a common technical problem during the spotting procedure, microarray data were not available for matrix metallo-protease-12 (MMP12), which in a previous study on the same SSc-MVECs was found to be up-regulated and responsible for urokinase-type plasminogen activator receptor (uPAR) truncation and subsequent angiogenesis impairment [ 18].
VCS-7 received training in defensive fighter tactics, aerobatics, navigation, formation flying and spotting procedures in Royal Air Force Spitfires; they flew spotting missions in the Spitfires because of the threat from German fighters.
Following a manual spot removal procedure and fusion of duplicate spot data (BlueFuse proprietary algorithm), the resulting fluorescence intensity data and quality annotations for the 17,102 gene features, were exported into the GeneSpring GX version 10.0.2 analysis platform (Agilent Technologies, Wokingham, Berkshire, U.K). after undergoing a block Lowess normalisation.
The full-length protein might have escaped the spot picking procedure since only a limited number of detected spots were further processed.
In particular, when the quality of the spot printing procedure is not constant (often the case with in-house spotted arrays), it is essential to account for an array effect in the ANOVA model [ 9].
Where possible, the same spot counting procedure, equipment and software (including settings) should be used.
To identify new DOX resistance genes, we utilized a "spot" testing procedure identical to that which we had previously used to identify IR resistance genes.
