Sentence examples for spotted separately from inspiring English sources

A 1 − μL aliquot of TLC Spot Q, R, and M methanol extracts were spotted separately with 1 − μL of a MALDI matrix solution of α - cyano-4-hydroxycinnamic acid (αCHCA, 5 mg/mL αCHCA in 50 50 (v:v) acetonitrile: 0.1 % (v/v) trifluoroacetic acid in water).

Labeled probes prepared from larvae that fed for 24 h on WT and the different, progressively defenseless plants were hybridized to microarrays on which normalized midgut-specific cDNA libraries of Ms and Hv neonates were spotted separately to identify differentially regulated genes (up-regulated: expression ratio >1.5; down-regulated: expression ratio <−1.5) (Figure 1A).

A peptide calibration standard ranging from 1046.54 to 3147.47 g/mol was spotted separately onto the MALDI target plate.

The collection was spotted onto YPD plates with deletion set strains ho Δ:: KanMX, lys2 Δ:: KanMX, and lys12 Δ:: KanMX spotted separately at the top and bottom of each plate as controls that do not affect spt10 Δ growth.

For the spot assay, nine serial three-fold dilutions were prepared for each strain starting with 6.6×10 cells/ml, so that the last spot contained approximately 50 conidia; 5 μl from each dilution was spotted separately on plates containing glucose-fructose-sorbose medium with agar (GFS) supplemented with the appropriate chemicals.

Randomly selected 150 plasmid samples of each library were spotted (approximately 50 ng plasmid DNA each spot) separately on 8" × 10" nylon membrane (N+, Amersham Hybond) in duplicate.

All approaches analysed the hybridisation signals for each spot separately and generated a cut-off per spot to determine whether a sequence was conserved or divergent.

One possible solution for this problem is to analyze the spots with oligos specific for T (T, TR, TG and TRG spots) separately from the ones with oligos not hybridizing with T genes.

Reproducibility of the spectrum was checked several times from separately spotted samples.

To check the reproducibility of the spectrum, sample was separately spotted several times.

Each prepared inoculums of C. albicans, C. tropicalis and C. glabrata were then separately spotted (40.00 μL) on the prepared culture plates.