As adults we can spot product placements for what they are – I hope you all spotted that I was paid to say Scotch tape rather than Sellotape just now – but children, by definition, are more naive.
On the team page, you can spot product designers, web engineers and mobile engineers who were all working for Number26.
Microarray were hybridized with 33P-labelled probes, first with an oligonucleotide sequence common to all spotted PCR products (5'TcalledGGAAACAGCTATGAC3') called vector probe to measure the amount of PCR products spotted onto the microarray.
Wisdom is easy after the event: would he have spotted such products before the crisis, asked one committee member.
Officers also spotted "cleaning products... dried blood spatter [and] a bucket of water with a chainsaw bar inside," according to a search warrant filed in the case.
This microarray consisted of duplicate spotted PCR products, representing all annotated genes in S. pneumoniae strains TIGR4 and R6.
The spotted PCR products were crosslinked to the slides at 450 mJ using a Stratalinker UV 1800 (Stratagene, La Jolla, CA, USA).
To verify the quality of spotting on the microarrays and the amount of DNA accessible for each spot, a vector probe (labelled oligonucleotide common to all spotted PCR products) hybridization was performed.
DNA microarray hybridizations were done as previously described [ 14, 15] on home-made nylon DNA microarrays (TAGC, Marseille-Luminy, France), which contained spotted PCR products from 945 human cDNA clones.
Microarrays were hybridized with P-labelled probes, first with an oligonucleotide sequence common to all spotted PCR products (5'-TCACACAGGAAACAGCTATGAC-3'), then after stripping, with complex probes made from 5 μg of retrotranscribed total RNA.
