Warm mackerel fillets on toast with piccalilli and a pear, walnut and blue cheese salad are a pleasant warm-up for fish pie packed with smoked haddock and prawns, and a spot-on medium-rare steak.
Equal amount of cells for each serial dilution were spotted on selective medium and pictures were taken after 3 days of growth on the selective medium.
The BX04 mutant cells harboring either pINIII-CFM4, pINIII-CspA (positive control), or pINIII (negative control) were grown in Luria-Bertani (LB) medium containing ampicillin and kanamycin, and the serial-diluted cultures (from 10-1 to 10-5) of BX04 cells were spotted on LB medium and incubated at low temperature (20°C).
For each mating, heat-shocked ascospores were spotted on sorbose medium and allowed to germinate at 22°C for 24 h.
For each mating, the haploid strain was spotted on rich medium plates (384 strains/plate), the deletion collection was spotted on top using a Singer Rotor robot (Singer Instruments, Somerset, UK), and plates were incubated for 24 hr at 30°.
Cultures were diluted to an OD600 of 1, serially diluted 10-fold, spotted on YPD medium with or without 2.5 or 5 µg/ml phleomycin, and grown for 2 3 d at 30° before imaging.
Overnight cultures were diluted to an OD600 of 0.01. 1 μL of this suspension was spotted on motility medium consisting of 0.3% agar, 1% tryptone, and 0.5% NaCl and incubated at 30°C for ∼14 hr.
These were serially diluted 1 10, 1 100, and 1 1000; 10 μl of these cell resuspensions were spotted on minimal medium containing different concentrations of glutathione (15, 30, 50, 100, 150, 200 μM) or methionine (200 μM) as sole organic sulfur source.
(A ) 10-fold serial dilutions of transformants of P GAL1-RPS5 histrain strain (JVY07) with the indicated plasmid-borne RPS5 alleles were spotted on synthetic medium supplemented with histidine and containing galactose (SGal + His + Ura + Trp) or glucose (SD + His + Ura + Trp) as carbon source and incubated at 30°C for 3 days (Glucose) or 4 days (Galactose).
