Sentence examples for spotted manually from inspiring English sources
For compounds showing reproducible activation, DMSO stocks were cherry-picked, diluted in DMSO, spotted manually onto assay plates, then assayed as above, albeit using manual solution transfers.
For replication experiments, compounds were cherry-picked from thawed DMSO stocks, spotted manually onto assay plates and processed as above.
The primary screen was performed in duplicates in 384-well plates (BD Falcon). 5 µl of each dsRNA tested (∼0.5 µg) was spotted manually at the bottom of the wells using an automated liquid dispenser (Multidrop, Thermo Labsystems) followed by the addition of 10 µl Fringe-GFP S2 cells resuspended in Schneider's Insect medium (Sigma) without serum at a concentration of 7×105 cells/ml.
The solutions of acetaminophen samples and acetaminophen standards (5 μL) were spotted manually on the chromatographic plates.
Probe sets designed from up- and down-regulated SSH libraries were spotted manually as macroarrays on nylon membranes and hybridized with different RNA targets.
The same mixture of four peptides was mixed with α-CHCA matrix solution at 3.5 mg/ml (1 1, v/v), and 1 μl was spotted manually 24 times on each of three MALDI plates.
We previously picked spots manually from normal Coomassie stained two-dimensional electrophoresis (2-DE) gels which were loaded with 500 µg protein [6].
Flawed spots (manually examined) and spots with more than 40% saturated pixels in any channel were removed from the analyses.
Background was automatically removed by the software on the basis of selected faint spots manually identified on the master gel.
We compared the Flamingo stained preparative gel with the DIGE master image, designated the differentially expressed spots manually in the preparative gel, and picked the protein spots with an automated spot picker (Bio-Rad).
Image analysis was carried out by placing the centre of each spot manually (grid-finding step) using the software AIDA (Raytest-Germany) and then by quantifying in a pre-defined neighbourhood around this spot centre using a two-dimensional Gaussian distribution (quantification step).
