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Using a multichannel pipette, 5 μl of Octodrine 10 mM was spotted directly on top of the C. albicans cells.
For the measurement of the clinical samples, WB was collected by finger prick and spotted directly on DBS cards.
A total of 0.5 µl of the sample was co-crystallized with the CHCA matrix and spotted directly on the MALDI AnchorChip target (Bruker Daltonics).
Desalted peptide solution (1 μl) was mixed with 1 μl of matrix solution (α-cyano-4-hydroxycinnamic acid in 0.1% trifluoroacetic acid and 50% acetonitrile) and was then spotted directly on the MALDI target.
An equal volume of α-cyano-4-hydroxycinnamic acid (CCA) matrix was added to the sample, and 1 μl of the sample mixture was spotted directly on a MALDI target for analysis.
Samples were eluted with 4 μl matrix solution (10 mg/ml CHCA in 50% ACN and 0.1% TFA) and spotted directly on a 384-well Opti-TOF MALDI plate (AB Sciex, Framingham, MA) in 1 μl aliquots and allowed to air-dry at room temperature.
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Reaction aliquots withdrawn at different time intervals were checked for methylation by capturing the biotinylated-methylated duplex DNA via avidin coated microplate and the unbound fraction by spotting directly on DE81 membrane.
One microliter of the digested sample was spotted directly onto a MALDI target plate and 1 μl of CHCA matrix solution was applied on the sample spot and allowed to air dry.
PCR products were checked for single products on ethidium bromide stained agarose gels and spotted directly.
For the growth competition assays, lysate or PBS (control) was spotted on a LS-LB 3% wt/vol agar growth plate, a nitrocellulose filter was placed on top, and indicated mixtures of cells were spotted directly over the lysate.
If Mr. Wyly could have given the money he spent on the television spots directly to the Bush campaign, the campaign alone would have been held responsible for any misleading information that might have been put out.
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