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Spots were stained using DAP that contained diphenylamine, aniline, acetone, and phosphoric acid (Merck KGaA, Darmstadt, Germany), and subsequently heated at 120 °C for 15 min.

Spots were stained using a Vectastain® Elite kit (Vector Laboratories, Burlingame, CA, USA) and counted by a computer-assisted video imaging analysis system (AID EliSpot Reader System, Autoimmun Diagnostika, Strassberg, Germany).

Following Ca overlay assay, spotted proteins were stained using Coomassie brilliant blue (see Figure  3C).

Nuclei were stained using TOPRO3.

Microtubules were stained using antibodies.

Sections were stained using hematoxylin-eosin.

Gels were stained using Coomassie blue.

Nuclei were stained using DAPI (Sigma).

Slides were stained using hematoxylin-eosin.

Collagen was stained using picrosirius red.

DNA was stained using Hoechst 33342.