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Membranes were exposed digitally using the ChemiDoc MP System (Bio-Rad), and spots were quantified using the ImageJ software.
Arrays were scanned and spots were quantified using Genepix 4000B with GenePix 6.0 software.
The screens were imaged on a Typhoon 9400 laser scanner (GE Healthcare, Freiburg, Germany) and the radiolabeled spots were quantified using Quantity One software (Bio-Rad, Munich, Germany).
Next each microarray was scanned using a GenePix 4000A laser scanner and the array features (spots) were quantified using the GenePixPro 5.0 and normalized using the Acuity 3.0 Microarray Informatics Software (Axon Instruments).
Images of the hybridized arrays were acquired using a ScanArray 4000 XL laser scanner (Perkin Elmer, Boston, MA) and fluorescent intensities from spots were quantified using GenePix 5 software (Axon Instruments, Inc., CA).
Spots were quantified using an adaptive method.
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The intensity of the spots was quantified using ImageJ.
The number of spots was quantified using a KS-ELISPOT-Reader (Carl Zeiss, Oberkochen, Germany).
The intensity of the spots was quantified using the ImageQuant TL software.
Pixel density of the spots was quantified using the ImageJ software.
The number of spots was quantified using an auto-analysing system, KS ELISPOT Compact (Zeiss, Göttingen, Germany).
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