Sentence examples for spots were performed using from inspiring English sources
The image analysis and calculation of mean background-subtracted intensity of the spots were performed using QuantArray software version 2.2 (PerkinElmer).
Tryptic digestion of interesting spots were performed using the ProteoExtract All-in-One Trypsin Digestion Kit (Merck Chemicals Ltd., Nottingham, UK) in accordance with supplier's instructions, with overnight incubation of samples at 37°C in the presence of trypsin final concentration of 4 ng μl-1 as the final step.
Identification of interested protein spots was performed using MALDI-TOF/MS MALDI-TOF/MS
Imaging of Cy3 and Cy5 labelled protein spots was performed using a Typhoon Imager 9400 (GE Healthcare).
Quantitation of the detected spots was performed using the Quantity One Software 4.4.1 (Bio Rad Laboratories Inc).
Acquisition of fluorescent spots was performed using the ScanArray Express software (Perkin-Elmer, Foster City, CA).
Spot quantification, visual inspection of potential outliers, and flagging of anomalous spots was performed using the program ArrayPro Analyzer (version 4.5; Media Cybernetics).
Image analysis and quantification of hybridization intensities for each spot were performed using the Xdots Reader program (COSE) and determined in pixels [ 39].
Spotting was performed using an Intelligent Automation Systems custom-built arrayer (Brooks Automation, Chelmsford, MA) with a 48-pin print head.
The marking of the M-SLN spot was performed using a skin-marker method [ 18, 19].
Spotting was performed using a non-contact array nano-plotter 2.1 (GeSim, Dresden, Germany) with a Picoliter pin that deposited 100 pl/spot.
