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Fluorescence intensities of array spots were measured using the ProScanArray Microarray 4-Laser Scanner (PerkinElmer) using the Blue Argon 488 excitation laser set to the FITC setting (494 excitation and 518 emission).
In order to quantify the differential expression of these receptors the densities of autoradiographic spots were measured using ArrayVision software (Table 3).
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The intensity of the spots was measured using a phosphor imager.
The area of fluorescent spots was measured using the Bio-Rad densitometer image analysis system (Bio-Rad, Hercules, CA, USA).
The intensity of 5-methylcytosine and cytosine mononucleotide spots was measured using a PhosphorImager screen and the Image Quant image analysis program (GE Healthcare).
The fluorescent intensities for each cDNA/gDNA spot were measured using GenePix Pro 3.0 software (Axon Instruments).
The intensity of the spot was measured using phosphor imaging with image gauge software.
For quantification of the fluorescence intensity of single YFP spots at septa, the intensity of the spot was measured using ImageJ (http://rsbweb.nih.gov/ij/).
The focal spot sizes were measured using a slit method with a CCD detector with a pixel pitch of 22 µm.
Relative spot intensities were measured using version 3.23 of the AIDA 2D densitometry software (Raytest, Straubenhardt, Germany).
Blue/purple spots, representing ASCs, were measured using an ELISpot reader and software (CTL Europe).
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