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Spots were developed using the alkaline phosphate substrate BCIP/NBT kit (Thermo Fisher Scientific).
Spots were developed using the Vectastain Elite ABC kit and Vector Novared substrate kit (Vector Laboratories Inc). according to the manufacturer's instructions.
Radioactive spots were developed using a Storm phosphorimager (Amersham Biosciences, Stockholm, Sweden) after 1 week of exposition, and the spot intensities were analyzed using ImageQuant Cell proliferation was measured by the MTT assay, as previously described [55].
After washing with tap water and 1 h incubation with streptavidin-AP conjugate (Dako) at room temperature spots were developed using alkaline phosphatase substrate BCIP/NBT (Sigma) and enumerated using a dissecting microscope.
Spots were developed using 5-bromo 4-chlorophosphate (BCIP; Sigma-Aldrich).
The spots were developed using freshly prepared substrate (ACE; Sigma, St . Louis MO, USA) dissolved in distilled water.
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Membranes were developed using ECL Substrate.
Bands were developed using enhanced chemiluminescence.
Blots were developed using ECL (Amersham).
Blots were developed using the Odyssey system.
An algorithm for burned area mapping in Africa based on classification trees was developed using SPOT-VEGETATION (VGT) imagery.
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