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Spots were detected using automatic spot detection and the background was subtracted using the mode of non-spot method.
Spots were detected using the default spot detection settings in the Delta2D analysis software (DECODON GmbH, Greifswald, Germany).
A total of 255 and 258 spots were detected using buffers with Triton X-100 or ASB-14, respectively.
The stained protein spots were detected using software Image Master (Amersham).
None of the selected spots were detected using antisera from naïve rabbits (not shown).
Positive spots were detected using an AID ELISpot reader system (AID Autoimmun Diagnostika, Strassberg, Germany).
Similar(43)
The intensities of the reflected light beams from the different spots are detected using a high speed area scan image sensor.
In each image, diffraction-limited spots are detected using band-pass filtering and refined using an iterative Gaussian mask localization procedure (Crocker and Grier, 1996; Thompson et al., 2002; Larson et al., 2005, 2011).
Twelve spots (including one major spot) were detected using a UV lamp (at 254 and 366 nm) and spraying sulfuric acid 20%% in ethanol followed by heating [41, 42].
Numbers of protein spot were detected using ImageMaster 2D-Platinum software version 6.0 (GE Healthcare).
Eight FSAs with high SIRs (hot spots) (M5C, M5E, M5G, M5M, M5R, M5S, M5T, M9R) and one FSA with a low SIR (cold spot) (M3H) were detected using the Getis-Ord Gi* method (Table 1, Fig. 6, Additional file 1: Legend 1).
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