Sentence examples for spots were destained using from inspiring English sources

Protein spots were destained using washing solution containing 50% methanol and 5% acetic acid in ultra deep water at 25°C for 18 h.

To visualize clear zones formed by cellulase positive strains the plates were destained using 1 M NaCl solution [34].

To quantify the staining, cultured cells were destained using 10% cetylpyridinium chloride (CPC) in 10 mM sodium phosphate, pH 7.0, for 2 h.

Gels were destained using HPLC-grade water with microwave heating.

Membranes were destained using 1 X TEN buffer for 10 min.

Excised gel bands were destained using 50% acetonitrile in 25 mM NH4HCO3, pH 8.4 and vacuum dried.

The gels spots were destained with 50 μ1 50%.acetonitrile (ACN) in 25 mM NH4HCO3 and dried using a speed vacuum for 10-15 min. To the dried spots, 10 mM DTT dissolved in 100 mM NH4HCO3 was added and incubated for 1 hour at 56°C.

Excised gel spots were destained with 100 μl of destaining solution (30 mM potassium ferricyanide and 100 mM sodium thiosulfate) with shaking for 5 min.

Subsequently, the transferred gel spots were destained in a destaining solution (100 mM Na2S2O3 and 30 mM K3Fe (CN)6, V/V, 1 1).

Excised protein spots were destained in a 1 1 mixture of 30 mM potassium ferro-cyanide and 100 mM sodium thiosulfate.

Briefly, silver-stained protein spots were destained with 15 mM potassium ferricyanide and 50 mM sodium thiosulfate (1 1) and washed with Millipore-Q water.