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After 24 h incubation at 37 °C, the spots were assessed using antibodies against IFN-γ, and then stained with AEC (3-amino-9-ethylcarbazole; Sigma-Aldrich, St Louis, MO, USA) staining solution.
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The degree of agreement between data from whole-tissue sections and the mean value of three spots was assessed using the Cohen's κ coefficient.
The peripheral visual field boundary and blind spot were assessed using a size I4e target.
The blind spot was assessed using I4e stimulus.
Bleeding and spotting days and episodes were assessed using the World Health Organization reference period developed by Belsey and Farley [ 22].
The second (from The Nederlands Kanker Instituut and designated the NKI cohort [ 22]) contained 295 cases that were assessed using Rosetta NKI spotted oligonucleotide arrays [ 23].
Scores range from 0 to 16. Vocabulary and verbal knowledge were assessed using a lexical decision task, the Spot-the-Word (STW) test [ 23].
Time trends were assessed using birth cohorts.
Potential interactions were assessed using logistic regression.
Differences between groups were assessed using ANOVA.
LC3 spot count was assessed using ImageStream software.
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