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After trypsin digestion, the peptides were extracted from the gel and analyzed by MALDI-TOF MS. Using the criteria described in Methods, we identified confidently 61 GA-responsive protein spots (Table 1).
Ovotransferrin was identified in 9 spots (Table S2).
We identified ovoinhibitor precursor in 13 spots (Table S2).
By using this double approach, 19 proteins were identified in 17 out of 22 spots (Table 1).
MALDI-TOF MS and MALDI-TOF MS/MS were applied to identify the proteins corresponding to these three spots (Table 2).
Comparison of western blots immunoprobed with different patient sera demonstrated clear differences in immune recognition with individual patient serum recognising between one and 41 protein spots (Table 2).
Similar(15)
The histone H2A_2 gene of Aspergillus fumigatus lacks intron at the position 17 (hot spot) (Table S1).
The maximum homing distance was 11000 m (Fig. 3), and the maximum homing speed of 50.51±9.07 m·min−1 was recorded from the 5000 m spot (Table 1).
Additionally, members of certain of the large gene families, particularly F-box, LRR and NB-ARC domain containing proteins were under-represented in the hot-spots and over-represented in cold-spots (Table 6).
Using the SaTScan programme, and adjusting for the size of the population and women's age, a total of 126 (19 % of the all STIs) of the women who tested positive for at least one STI during the follow-up period were geographically clustered into five hot-spots (Table 2).
Fourteen proteins which had significantly altered expression were identified, where some of these proteins are represented by more than one protein spot (Table 3).
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