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Normalization of each individual spot was performed according to the total quantity of the valid spots in each gel, after subtraction of the background values.
The marking of the M-SLN spot was performed using a skin-marker method [ 18, 19].
The IR spectroscopy analysis of the active spot was performed using KBr plates (JASCO FT-IR Model-420) with a scanning speed of 2 mm s-1.
In addition, a near point lockmass correction for each sample spot was performed using adrenocorticotropic hormone fragment 18 39 (MH+2465.199) to achieve maximum mass accuracy.
In the event of discordant results between the 2 tests, DNA PCR by using dried blood spot was performed at the National Reference Laboratory.
Normalization using the lowess function, which computes the logarithmic intensity ratio (m) and the logarithmic mean signal intensity (a) for each spot was performed and t-test statistics was accomplished with the EMMA microarray data analysis software [ 51].
Similar(52)
Image analysis and quantification of hybridization intensities for each spot were performed using the Xdots Reader program (COSE) and determined in pixels [ 39].
Visualization of spots was performed with UV light and by treatment with iodine.
Identification of interested protein spots was performed using MALDI-TOF/MS MALDI-TOF/MS
Subsequently, the separation of the protein spots was performed for 40 min at 180 V.
Culture of the plates, washing, counterstaining, visualisation and analysis of the spots was performed according to the manufacturer guidelines.
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