Sentence examples for spot visualization from inspiring English sources

The reaction monitoring was carried out by Thin Layer Chromatography (TLC) using as mobile phase DCM: methanol (MeOH) = 9.5: 0.5 (v/v) and the spot visualization was done under UV light at 254 nm.

TLC plates (Yantai E.S.T. Silicone Tech Co., Ltd., China) consist of high-performance silica gel 60-F254 platesilicaica gel particle size 8 ± 2 μm ≥80 %, layer thickness 0.2 ± 0.03 mm) with glass back plates.The plate containing a fluorescing additive, F254, was used for easy spot visualization.

Gels were fixed in solution containing 25% (v/v) methanol and 10% (v/v) acetic acid for 1 hr and stained using colloidal Coomassie Blue for spot visualization.

Spot visualization was completed by mildly heating the plate with an air dryer.

The spot visualization feature in BASE was employed for an additional quality control for false positives/negatives.

However, changes in expression of these genes were either verified by qPCR and direct spot visualization in BASE or were present in our comparative analysis of our microarray data with data published by van der Graaff et al. [ 22].

Raw data were extracted from the DNA microarray hybridization images with GenePix Pro 5. Spot image visualization, spot quality evaluation, data normalization, and SAM test for the identification of the differentially expressed genes were performed as previously described using the RealSpot software [ 61].

Image visualization, spot detection, and protein quantification were carried out using the Melanie 3 software (GeneBio, Geneva, Switzerland).

ImageQuant version 1.2 (Molecular Dynamics) was used for visualization and spot finding, spot quantification.

Corresponding peaks are easily spotted with this visualization, as well as peaks that are only present in one of the chromatograms.

For visualization and spot isolation of plasminogen-binding proteins, blots were stained with India Ink.