In many studies, a single spot tissue specimen was obtained.
Investigated TMAs contained samples of kidney cortex (6 caspots spots), bladder (5 caspots spots) and prostate (6 caspots spotissuessues, respectively.
Such findings concerned six analyzed tissue types: lymph node (11 caspots4 spothyroidyroid (5 caspots skeletalskeletal muscle (1 caspotsspleen, spleen (1 caspotsspots), diaphragm (1 caspotsspots) and adipose (1 caspotsspotissuessues.
The subsequent RT-PCR experiments from egg-spot tissue of A. burtoni were positive for the xanthophore-related color gene csf1ra.
We amplified and sequenced a 540-bp fragment of csf1ra from cDNA that was transcribed from mRNA extracted from A. burtoni egg-spot tissue, using the primers F_1986 5'-GCTGCCCTACAATGAAAAGTG-3' and R_2186 5'-TTGACGATGTTCTGGTGGTGA-3'.
Here, we show that the egg-spots of haplochromines are made up of yellow pigment cells, xanthophores, and that a gene coding for a type III receptor tyrosine kinase, colony-stimulating factor 1 receptor a (csf1ra), is expressed in egg-spot tissue.
CMC cultures, analysed after in vitro enrichment in stem-like cells, display CD44 expression in a high number of cells, while, in the tumor of origin, CD44+ cells are confined in randomly spotted tissue areas, as described for human samples [ 71], explaining, as previously discussed, the apparent high percentage of CD44 negative tumors.
This procedure was justified by looking at 30 measurements of each spot and tissue type, which showed only minimal variation, and 6 measurements of each animal and tissue type at its different spots, which show almost exclusively only a constant vertical shift.
Electron microscopy of these fields revealed a spot of tissue damage presumed to be associated with the implantation site of the carbon fiber microelectrode.
Using ImageJ-based macros, regions of interest (ROI) were selected for each tissue microarray spot to exclude folded tissues and inappropriate tissue regions [ 52].
