Sentence examples for spot expression from inspiring English sources

To construct a strain with arabinose-regulated spoT expression, a TT araC PBAD promoter cassette was inserted in front of the spoT gene.

Autoaggregation was, however, eliminated by the addition of 0.4% arabinose, indicating that a higher level of spoT expression was required to alleviate this phenotype.

There are many ecotypes of A. thaliana that differ widely in genotype and phenotype: as expected, this extends to differences in both protein spot expression and protein spots identified in root proteomes [8].

To investigate the contribution of ppGpp to the virulence of Y. pestis, we infected groups of three Swiss Webster mice subcutaneously with wild-type, χ10003 pCD1Ap) (ΔrelA233), χ10004 pCD1Ap) (Δreland3 ΔspoT85) and χ10019 pCD1Ap) (ΔrelA233 ΔspoTT5 ΔlaraC16::TT araC PBAD spoT), in which spoT expression is regulated by arabinose availability.

We chose the top ten spot expression profile groups (with distance parameters less than 0.5) with almost identical expression profiles.

Differences in protein spot expression levels were considered as statistically significant when t-test resulted in a P-value<0.05.

We found that the small gel format was of sufficient resolution for cluster analysis to be performed, resulting in the separation of normal and benign breast tissue from breast cancer specimens on the basis of their protein-spot expression patterns.

While the quantitative analysis of the spots' expression showed that a total of 19 stained protein spots (Fig.  1) showed significant changes of at least threefold up- or down-regulated expression in the healthy group (Fig.  2).

GAPDH has previously been shown to be post-translationally modified as reviewed in Sirover and Butterfield et al. Interestingly, in the comparisons of the two DLBCL presentations (analysis C), the GAPDH-positive spots showed high expression (spot 8403) or low (spot 7305) expression in secondary DLBCL.

Being R and G, the background-corrected red and green intensities for each spot, the expression log-ratio (M-value) corresponding to a spot is M = log2R - log2G, whereas the log-intensity (A-value) of each spot is defined as A = (log2R+log2G /2, a measure of the overall brightness of the spot.

A paired t-test was used to select 22 spots whose expression variation between both cell types was statistically significant (Student's t-test, p<0.05).