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Khamis Kagasheki's proposal for perpetrators of the illicit ivory trade to be executed "on the spot" divided opinion, with some conservationists backing it as a necessary deterrent but others warning that it would lead to an escalation of violence.
Proteins were ranked based on mean Cy5 of a spot divided by normalized Cy5 background signal as a proxy for amount of protein present in each spot.
Each spot (divided into 100 positions) was measured from positions 20 to 80 with steps of four positions.
The resulting image was quantified and the intensity of each spot divided by the median spot intensity to provide a scaled and comparable number across multiple arrays.
The miRNA microarray data were quantified by the absolute fluorescence intensity of a spot divided by the median fluorescence intensity of all spots and normalized against the internal control U6 gene.
The normalized value of a spot was calculated from the spot signal minus the background level of hybridization (determined from the intensity of the signal surrounding each spot), divided by the median value of all the signals detected on the filter.
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The percentage of high quality spots (SNR > 10) were calculated as the number of high quality spots divided by the total number of spots on the array.
The number of PLA spots divided by the number of nuclei was obtained from each image, and mean values ± the standard error of the mean (SEM) of the ratio was defined as PLA/cell.
To measure the volume and intensity of protein spots on CBB-stained gels the volume and intensity of each spot was divided by the total volume or intensity of all spots of the same gel.
The spots once divided into several spots and then concentrated again into a spot per nucleus in the dividing nuclei of anaphase.
In this analysis, the SAM patterns that developed from an initial CZ spot are divided into six groups according to their structure and proliferation (Fig. 3).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com