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An alternative technique that could prove to be a useful tool in the study of the histone code is the use of synthetic peptide arrays (SPOT blot analysis) as a screening approach to characterize macromolecules that interact with specific covalent modifications of histone tails.
Samples were clarified by centrifugation and analyzed by quantitative spot blot as described above.
Quantitative spot blot results for batch production were normalized to the highest producer (L).
From 32 positive cells and/or colonies picked, 8 showed strong growth and they were analyzed by spot blot.
The purified IGF-E5 was then used to make a standard curve for quantification by spot blot (see below).
bProduction from a 3-day batch culture at 30°C was measured by quantitative spot blot against purified IGF-E5.
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The surviving 31 clones were first screened by spot blotting 3-day old culture supernatants.
Quantitative spot blots were made from batch samples, from clones seeded at 5 × 105 cells/ml and grown for 3 days (CHO-SF) or 5 days (293SF) under identical conditions at 30°C.
To more precisely identify the Bves-Bves interaction region in the C-terminus, a SPOTs blot membrane was generated by SIGMA-GENOSIS (Woodland, TX).
if you have oily or shiny spots: blot gently with a clean facial tissue, and resist the urge to over wash your face (it will create more oil).
SPOT-blot peptide arrays were used to screen for the methyllysine-containing histone peptides that bind to MBT domains found in nine human proteins.
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