Spot assay to assess growth of RN1008 and related strains on various types of solid media.
A spot assay was carried out with various types of solid media plates.
To determine whether RN1008 was able to grow on other Pi sources, a spot assay was performed in which 1 mL of RN1008 culture grown on MOPS-Pt at late log phase was centrifuged, washed once with an equal volume of MOPS-0, and diluted using a 10-fold series by 107-fold.
This study aimed to evaluate the agreement between blood spot and plasma chitotriosidase using the economic substrate 4-methylumbelliferyl-β-D-N,N′,N″-triacetylchitotrioside, and to investigate the utility of the blood spot assay for the wide scale screening for lysosomal storage disorders among the clinically suspected.
Chemotaxis is frequently inferred by determining how many cells cross a boundary in a chemotaxis assay, for example how many cells crawl into a chemoattractant-infused filter, or how many cells enter a defined region in an under-agarose assay or agarose spot assay.
Also, both consensus and mosaic immunogens induced significantly higher gamma interferon (IFN-γ) enzyme-linked immunosorbent spot assay (ELISpot) responses than B.1059 immunogen.
Over 90% of study participants had no positive gamma interferon (IFN-γ) enzyme-linked immunosorbent spot assay (ELISpot) responses to any peptide pool at any time point.
The ER HAA1-OP strain showed not only higher acetate tolerance by spot assay but also higher fermentation ability (ethanol production) in the presence of 0.5% acetate than the wild-type strain.
It was carried out by spot assay in duplicate onto YPD plates containing Kan within various concentrations of ethanol 1, 3, 5, 7, 10 and 15% (v/v), respectively.
The spot assay results were confirmed by microtiter assays as well.
To assess LL-37 candidacidal activity, a spot assay and the FUN-1 staining assay were used.
