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The mixture of E. coli ET12567/pUZ8002 cells and heated spores were spread on MS agar plate supplemented with 10 mM MgCl2 and apramycin at 30°C overnight.
The spores were spread on a cover glass coated with Stay-on adhesive (Surgipath), coated with gold by IB-2 ion coater (Eiko Engineering), and observed with a TM3000 tabletop SEM (Hitachi).
Dilutions of Streptomyces spores were spread on R5 medium containing various concentrations of phenolic compounds.
Wild type S. coelicolor spores were spread on a YEME agar plate, pH 7. A filter paper containing only surfactin (50 μg) or a filter paper containing both surfactin (50 μg) and catenulipeptin (25 μg) was placed in the center of the plate.
Similar(56)
One hundred microliters of spore solution was placed on nine standardized 60 mm Potato Dextrose Agar plates (PDA, 24 g/L Potato Dextrose, 15 g/L Agar, 50 mg/L kanamycin) per isolate and spores were spread with a sterile glass rod.
Spore lawns of M600 and M570 were spread on SMMS agar plates [ 81], and paper discs impregnated with vancomycin (or blank) were immediately applied to the surface.
Fungal spores are spread by air currents and once they land on a leaf surface and optimal growth conditions are reached, they begin to germinate and form appressoria.
Truffles rely on being unearthed and eaten by animals so that their spores are spread in their dung.
Each suspension was evenly applied using a pressurized hand pump sprayer at the rate of 0.23 l kg−1 pumice stones, which were spread on a plastic sheet (0.47 × 1012 CFUs or spores kg−1 pumice stones).
A 25-μl aliquot of the spore suspension was spread on PDA.
A 10 μl aliquot of the diluted spore suspension was spread on PDA (Becton, Dickson and Company, Sparks, MD) medium.
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