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Red spores were selected on solid SD-Ura media.
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In order to mitigate decontamination test costs and schedule associated with BSL3 testing of B. anthracis Ames in a large-scale design of experiments, B. anthracis ∆Sterne and B. thuringiensis Al Hakam spores were selected for decontamination testing that measured decontamination on greater than 2,500 tests and controls with ≥7 logs of spores per test (Buhr et al. [2012b]).
Two haploid ho spores were selected: 2029-C5 froMTF202929 and 1782-B1 froMTF178282.
The transformants were selected on potato dextrose agar (PDA) (ATCC medium 336) supplemented with 50 μg/mL hygromycin and purified to uninuclear clone by diluting spores on PDA plates.
Spore was selected as the original host and the cell wall was eliminated after 4-h degeneration with enzyme mixture.
pRS416-TAH18-URA3 was chased from the diploid strain before sporulation, and a Δtah18:: TRP1-CYH2spore2Δ:: TRP1-CYH2S pRS415-DRE2TAH18 spore was selected.
The metal ions that showed a significant positive influence on spore production were selected for optimization by a central composite design (CCD) experiment and response surface methodology (RS M analysis.
After sporulation, segregants were grown in minimal medium and spores still carrying the GCR were selected.
The spores without bacterial growth around them were selected for extraction.
After 1 week, germinated spores that had developed considerable hyphal growth were selected for bacterial inoculation.
The strain showed good sporulation and high spore viability and hence, was selected for further analysis.
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