Sentence examples for spore extraction from inspiring English sources

The sampled roots and soils were transported to Mekelle University laboratory for estimating root colonization and spore extraction.

This method also permitted spore extraction in the test tube without extra handling steps of the spore-inoculated substrate.

For spore extraction, the soils first air-dried and pass through 0.75 mm sieve and weighed using sensitive balance to get a subsample of 25 g.

Wet spore controls, incubated at 22 ± 3°C and ambient relative humidity, served as the 100% spore recovery reference values in order to correct for spore extraction efficiency from different substrates.

Spore suspension of Aspergillus awamori was prepared by spore extraction with 10% (w/v) glycerol from a culture grown for 7 days at 30°C on 1.5% (w/v) corn meal agar.

Root-free fungal compartments of all plates were pooled per single spore line for extraction of hyphae and spores.

Careful centrifugation, pelleting, and rinsing of infective spores during RNA extraction, as well as PCR amplification using host-specific primers, has been used to ensure purity of isolation [ 12, 21].

For genomic DNA extraction, spores were inoculated in potato dextrose broth and grown at room temperature in a tissue rotator; after 3 days' growth, the mycelium was harvested and ground in liquid nitrogen.

Experimentally controllable parameters included in our simulation were population size, intensity of phenotypic selection, sequencing depth, and number of generations between spore isolation and DNA extraction.

Vortexing swabs for 2 min during processing resulted in superior extraction of spores when compared to sonicating them for 12 min or subjecting them to minimal agitation.

The growing medium containing mycelium and spores was dissolved in extraction buffer (0.82 mM sodium citrate and 0.18 mM citric acid).