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This is the first study to describe the enzymatic properties of the consensus integrase of HIV-1 clade A and the effects of the resistance mutations when the complex actions of sporadic sequence polymorphisms are excluded.
The percentage of "sporadic sequence dropouts" or failed reads that inevitably occur within a pool of high quality data is often overlooked and rarely examined.
The small number of individuals used for SNP discovery was chosen to capture most of the common SNPs and to reduce the amount of sporadic sequence errors interpreted as putative SNPs.
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Interestingly, we found that sporadic sequences from Burmese IDUs are derived from Burmese LDTDs, suggesting that Burmese LDTDs spread HIV-1 to IDUs.
While high-throughput centres report failure rates typically on the order of 10%, the causes of sporadic sequencing failures are seldom analyzed in detail and have not, in the past, been formally reported.
A Failure Mode Analysis (FMA) strategy was developed to determine the likely causes of sporadic unsuccessful sequence reads.
Two resequencing efforts (data not shown) detected only sporadic coding sequence variation among the set of cases and controls chosen for study, identifying no variation frequent enough to be responsible for the observed effects.
Representative histopathology of sporadic hemangioblastoma sequenced in this study.
YW participated in screening of the sporadic cases and sequence alignment.
The fighting in "Time" occurs in sporadic, hopped-up sequences that almost seem to erupt out of the star Brigitte Lin's luxuriant subconscious -- or out of the director's, since the camera gazes at her with a dizzy, barely conscious longing and melancholy that have become Mr. Wong's most recognizable asset.
Additionally, nested RT-PCR was conducted with ORF2-specific primer pairs (6 ) for 2 HEV isolates, 1 each from outbreak and sporadic cases, and sequences were obtained.
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