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In two other lumB families all members were classified as non- BRCA2-like (sporadic-like), indicating that BRCA2 is less likely to be involved in the development of cancer in these families.
The evolution of the variant Creutzfeldt-Jakob disease (vCJD) epidemic is hazardous to predict due to uncertainty in ascertaining the prevalence of infection and because the disease might remain asymptomatic or produce an alternate, sporadic-like phenotype.
Two of the discordant tumours tested Sporadic-like in both the MLPA and the aCGH assay, whereas six of the BRCA1-mutation carriers were either classified Sporadic-like by MLPA or aCGH.
These 'positive' samples were compared with breast tumours that were known to have a sporadic-like profile by aCGH.
The tumours with a Sporadic-like profile do not show a significant difference between conventional and intensive chemotherapy.
Among the lumB subtype, 7 samples were classified as BRCA2-like (39%) and 11 as sporadic-like (61%).
We used Shrunken Centroids from the package PAM to discriminate between BRCA1-like and Sporadic-like tumours.
If the tumour shows a Sporadic-like profile, there is still the possibility that the tumour would be BRCA2-like at aCGH.
Interestingly, one tumour which was classified as Sporadic-like by both MLPA and aCGH was hormone receptor positive, whereas all other BRCA1-mutated tumours were triple-negative.
Seven basal-like tumors out of nine were classified as BRCA1-like (78%) and the remaining 2 as sporadic-like (22%).
Three BRCA1-likeaCGH tumours were classified as Sporadic-like by MLPA and two Sporadic-likeaCGH tumours were typed as BRCA1-like.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com