By failing to repair spontaneous errors that occur during replication, these tumors accumulate frameshift mutations that affect tumor suppressor genes containing coding repeat sequences [1].
Non-PCR based template amplification in combination with ligation of growing DNA strands reduces the rate of spontaneous errors.
Similar to spontaneous nucleotide mutations in DNA sequences, spontaneous errors can also occur in epigenetic modifications (epimutation) (Becker et al. 2011; Schmitz et al. 2011; Schmitz, Schultz, et al. 2013).
Among reactions contributing to this damage, reactions of free radicals and other reactive oxygen species are the main reason, apart from reactions of metabolites such as sugars and reactive aldehydes and spontaneous errors in biochemical processes [ 9].
Important sources of genomic alterations are mutations, or spontaneous errors of DNA replication, [ 37, 38] that occur despite the existence of a wide range of mechanisms ensuring DNA repair and correct replication [ 39].
The imbalance could arise from any of a range of deficiencies or spontaneous errors, but it is appealing to consider the decatenation checkpoint because of its deficiency in wild-type stem and progenitor cells.
The predominance of transition mutations suggests that TP53 mutations in ovarian carcinomas arise because of spontaneous errors in DNA synthesis and repair, rather than the direct interaction of carcinogens with DNA.
Most outliers in regular microarray were the results of spontaneous experimental error, with low spot intensities.
With his discovery of primary nondisjunction, Bridges revealed for the first time that meiosis is an imperfect process subject to spontaneous, unprovoked errors.
Two different PCR reactions were made and sequenced for each patient to exclude that heterozygotes were due to rare spontaneous polymerization errors of Taq activity.
