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At a speciation event (internal node on the species tree; Figure 1) travelling past to present, the population splits into two isolated populations.
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The second branch splits into two main clusters, one corresponding to isolates that totally or partially lack cagPAI genes mostly associated with gastritis and the other clustering isolates associated with other diseases.
Typically, one species splits into two new species only when some of its members wind up isolated in a different location.
They are split into seven genetically isolated populations in Colorado, one of which includes a tiny group in adjacent eastern Utah.
CC199 splits into two main clades, one of which consists of all but one of the disease isolates, while the other comprises all but one of the carriage isolates (Figure 1).
That splits into two challenges.
The tunnel then splits into two.
This central cluster splits into two clades.
These susceptible isolates were split into two clades: ST236 isolates of serotype 19F (predicted to be the the ancestral serotype) formed clade "19F-βLS," and serotype 19A ST202 and ST3559 isolates formed clade "19A-βLS".
The other four isolates had unique restriction profiles and could be split into two groups of two isolates, based on the similarity of banding patterns.
The PBMC sample was split into two aliquots and CD14 monocytes were isolated from one aliquot and CD19 B cells from the other by magnetic cell sorting using CD14 and CD19 microbeads (Miltenyi Biotec) according to the manufacturer's instructions.
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