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The Hey (Hairy and Enhancer of Split related) family of basic-helix-loop-helix transcription factors is thought to mediate Notch signaling in the developing cardiovascular system [13].
This triggers the expression of downstream target genes such as Hes/Her (hairy/Enhancer of split related) family of bHLH transcription factors, which in turn modulate the expression of downstream genes and themselves [16], [20] [26].
HEYL, (Hairy/enhancer of split related with YRPN motif-like protein) is one of a group of transcription factors that act to inhibit gene expression and are thought to be involved in mediating Notch signalling, possibly with a role in epithelial-to-mesenchymal transition [46].
Underestimating the number of clusters can artificially group unrelated elements while overestimating can split related groups into subgroups that confound further analysis.
Although these 9 genes showed similar patterns, cold inducible RNA-binding protein, enhancer of split related 10, and tetratricopeptide repeat protein 30 showed opposite expression patterns between Salam and Drago treatment groups.
Three genes, cold inducible RNA-binding protein (5.7 fold reduction compared to control), enhancer of split related 10 (5.9 fold reduction), and tetratricopeptide repeat protein 30 (6.6 fold increase) showed the reverse expression profile in tadpoles exposed to larval salamanders compared to dragonfly larvae.
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The miR-1281 target HEYL (hairy/enhancer-of-split related with YRPW motif-like) is associated with HCC [ 62, 63].
In Notch pathway, we focused on two genes, Notch1 and Hairy/enhancer-of-split related with YRPW motif protein 1 (HEY1).
Hairy/enhancer-of-split related with YRPW motif 1 (Hey1), as well as its activator Notch1, were more abundant in the ventricles than in the atria.
Among these, GPR37 (G protein-coupled receptor 37), HEYL (hairy/enhancer-of-split related with YRPW motif-like), DNJA1, CD14 (Cluster of differentiation 14) and GNS (glucosamine (N-acetyl -6-sulfatase) are kN-acetyl -6-sulfatase N-acetyl -6-sulfatasec disorders.
This network contained a number of transcription regulators, including BTG2 (BTG family, member 2), EGR1 (early growth response 1), FOSL1 (Fos-like antigen 1), HEY1 (hairy/enhancer-of-split related with YRPW motif 1), NFATC1 (nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1) and NFKB1 (nuclear factor of kappa light polypeptide gene enhancer in B-cells 1).
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