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The first stage was that ancestral population split into two populations of QIN and non-QIN.
We assume that an ancestral host population has split into two populations, a large mainland and a small island.
At day 4 the cells were split into two populations; one was kept at 32°C and the other was shifted to 39°C.
The computer program IMa [36] was used to fit an isolation-with-migration model, which assumes that an ancestral population of size θA split into two populations of sizes θ1 and θ2, t generations ago, and that after divergence the two populations receive immigrant genes from each other with rates of m1 and m2.
The digested cDNA was purified and split into two populations before ligation of Adaptor 1 or Adaptor 2R.
Subsequently, the larvae were split into two populations and transferred to vials with yeast concentrations of 100 g/l and 10 g/l, respectively.
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The total sample was artificially randomly split into two population subsamples of equal sizes (nA = nB = n/2) as if they came from different geographical location though all the simulations involved a single well mixed population (without any limitation of flow between the subsamples).
Then, 420 generations before present the ancestral population splits into two populations.
In divergent selection experiments, the initial inbred is splitted into two populations, that are artificially selected for highest and lowest values of a given trait.
IMa is based on a Bayesian coalescent implementation of a model in which an ancestral population splits into two populations that may exchange genes in both directions at unequal rates during divergence.
An ancestral population with size N A increases its size to N B at time t = 0; the population size remains constant and equal to N B up to time t = t1; at time t = t1 the population splits into two populations with sizes N1 and N2; the present time is reached at time t = t2.
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