Sentence examples for split into two flasks from inspiring English sources

Exponential cultures were split into two flasks.

The early-passage primary culture was split into two flasks and the cells were allowed to grow for different lengths of time.

The culture was split into two flasks and methionine was added to a final concentration of 60 ng/ml, of which 0.5 ng/ml was [S] methionine (cell-labelling grade 1175 Ci/mmol; New England Nuclear, Boston, MA).

Cells (1 × 10) were split into two flasks and after further growth, were subsequently harvested in either an exponential or confluent, growth arrested state (each flask after different lengths of time in subculture).

This labelling solution was then added to each well of the cells and incubated for 1 hour at 37°C after which they were washed twice with fresh media and split into two flasks.

Four independent cultures (biological replicates) were grown in Friis broth [ 23], each split into two flasks (technical replicates), until the media color change indicated mid to late log phase of growth had been achieved (pH ~ 6.5).

The culture was then split into five flasks and fresh medium and blood was added to obtain a hematocrit of 5%.

At this time, the cells were lifted by trypsin/EDTA and split into two 75 cm2 flasks containing fresh medium.

Each clone was split into two parallel flasks, in one of them cells were grown without doxycycline.

A single 175 cm flask of RK-13 cells was grown to confluence (defined as a continuous cell monolayer) and split into three separate flasks.

At the fifth passage, each cell line was split into three parallel flasks designated biological replicate 1, 2 and 3, and grown to approximately 70% confluence.