The first stage was that ancestral population split into two populations of QIN and non-QIN.
We assume that an ancestral host population has split into two populations, a large mainland and a small island.
At day 4 the cells were split into two populations; one was kept at 32°C and the other was shifted to 39°C.
The computer program IMa [36] was used to fit an isolation-with-migration model, which assumes that an ancestral population of size θA split into two populations of sizes θ1 and θ2, t generations ago, and that after divergence the two populations receive immigrant genes from each other with rates of m1 and m2.
The digested cDNA was purified and split into two populations before ligation of Adaptor 1 or Adaptor 2R.
Subsequently, the larvae were split into two populations and transferred to vials with yeast concentrations of 100 g/l and 10 g/l, respectively.
One scenario is that a contiguous ancestral population was split into two populations by the rise in sea level, leaving two disjunct populations, one in the then Tombigbee River and the other in the then Chattahoochee River.
After the onset of speciation, however, a species will be split into two populations that are completely separated and will thus form clusters separated by a gap exceeding 2 Ne.
Two 10 cm plates of adult animals were harvested by washing with S-Basal and split into two populations: ∼50 animals were lysed in 40 µl of single worm lysis buffer for gDNA preparation, as described above; the remaining animals were frozen at −80°C for total RNA preparation.
Approximately 200 adult worms from an individual transgenic line were split into two populations; one group was exposed to S-basal buffer alone (to test for chemotaxis), while the other was pre-exposed to the AWC-sensed odor, butanone, in S-basal buffer for 80 min under otherwise identical conditions (to test for adaptation).
