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The majority of the split FISH signals are most likely due chromosome translocations, rather than persistent DSBs.
Since Ku, required for the tethering of DNA ends of DSBs, is functional in AT5BIVA and 11-4 cells, we speculate that most of the MLL gene rearrangements detected as split FISH signals are chromosome translocations [26].
Split FISH signal (separated red and green signal) indicated a rearranged RET gene.
Split FISH signals were absent in all negative controls (cell lines without RET/PTC rearrangement or with expression of the wild-type RET).
Fusion and split FISH analyses using probes for EML4 and ALK genes confirmed that the adenocarcinoma of the right lung was indeed EML4-ALK positive.
A split FISH signal in red and green within a cell nuclei in addition to an overlapping signal (mixed colour, yellow) indicates a rearranged RET gene, whereas normal cells show two overlapping signals.
Similar(51)
More fishing means that jellyfish will more frequently clog up, and split, fishing nets.
To monitor sister chromatid separation in further detail in MM, we used the centromeric probe system described above and selected late metaphase/early anaphase cells for analysis in which at least one pair of sister centromeres in the metaphase/early anaphase plate had separated, as evidenced by split FISH-signals in a double-dot formation.
But she has done little to rein in construction costs for the Olympics, and after several months of study, she decided to split the fish market, moving wholesale operations while renovating the old site so its restaurants and shops could remain for tourists.
Having dinner at Cecconi's restaurant in West Hollywood on April 8, Simon and Mezhgan each had a green salad, and then they split a fish entree, avoiding the pastas and desserts.
This night we split the fish special, a piping-hot fresh branzino, plump and juicy with a crispy fried outside on a bed of tummy-warming mashed potatoes and spinach ($24), so good with a Maremma Super Tuscan to wash it all down ($10).
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