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The liquid broth from the fermentation might be split by filtration or centrifugation in a solution and in a cell concentrate.
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This process removes the salt by filtration, using membrane technology.
For LXR activity measurements on minimal medium, 100 mL of medium containing 1% (w/v) glycerol was incubated for 24 h, and the mycelia were collected by filtration, split into two comparable portions, resuspended in 50 mL of MM medium supplemented with either 1% d-glucose or 1% l-arabinose, and incubated for 15 h at 28 °C.
Overnight (16 h) growth at 28 °C resulted in a dense homogeneous mycelium suspension, which was collected by filtration and split into two comparable portions.
After purification by centrifugation and disruption with sodium deoxycholate, the split virus was homogenised and sterilised by filtration.
The split virus was further purified by ultrafiltration and sterilised by filtration.
Primary urine is formed by filtration from the blood.
The lysate was sonicated, centrifuged and clarified by filtration.
Formaldehyde was removed by filtration using Amicon Ultra Centrifugal filter units (Millipore).
Samples were concentrated by filtration, cleaned up by acetone precipitation and quantified.
The resulting precipitate was collected by filtration, dried overnight and subjected to aminolysis as below.
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