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A single C>T base change in exon 7 of SMN2 results in abnormal splicing with the majority of transcripts missing exon 7 that encodes a crucial oligomerisation domain, and therefore results in a non-functional protein.
Like most other CGs, alternative splicing with the inclusion of novel exons was also observed in these test cases.
In addition, by RT-PCR, analyses of several human caspase genes indicate that these In100-containing caspase genes indeed undergo alternative splicing, with the skipping of exons immediate upstream of their In100-like elements.
To evaluate the relationship of altered GBP5 splicing with the SSC4 QTL genotype, all RNA-seq reads that discriminate the three alternatively spliced transcripts were counted.
A variant predicted to be leaky exhibited residual normal splicing, with the exception of cases where a much stronger cryptic splice site was activated; c.
Several of these changes occur at the level of alternative splicing, with the generation of variants that promote multiple aspects of tumorigenesis.
Similar(43)
Therefore, we initially examined SMN2 trans-splicing with the goal of devising strategies to enhance trans-splicing efficiency in vivo.
The specificity of trans-splicing with the intended target splice site was improved 10-fold by designing tsRNA that harbors secondary target binding domains shielding alternative on-target and blinding off-target splicing events.
But there were more venomous comments, spliced with the darkest humour, after the energy supplier invited users of Twitter to raise questions with its customer services director.
Spliced with the clinical language of psychopathology, representations of individual incidents of violence (self-harm, rape, murder) can distract from the structural, historical causes of such violence.
According to the Pittsburgh Post-Gazette: remixed Dolly Parton spliced with the optimistic ejaculations of Carnegie Mellon grads, with just a hint of irony.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com