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The HiBi-PCF was completely collapsed near the splicing points, thus the cladding mode in the HiBi-PCF would be excited, which was sensitive to the refractive index (RI) of surrounding medium.
These events include the start and end positions of exons as well as the continuation of the exon at all intermediate introns splicing points.
After FDR controlling, the peptides crossing the Fusion or Splicing points were extracted as candidates.
We introduce the flow difference metric (FDM) to measure the difference between two graphs in the relative utilization of edges at splicing points.
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These findings contribute significantly to the toolbox of intein-based technologies by allowing the use of inteins in proteins having proline at the splicing point.
As a result of alternative splicing, point-mutations, degradations and co- and post-translational modifications, the number of protein species [1] of a proteome exceeds by far the number of protein-coding genes of the corresponding genome.
A 45-kD soluble form of MMP16 has also been suggested to be formed by alternative splicing of mRNA [11], the splicing point potentially lying in the hemopexin-like domain.
The PCF in the sensor head has a microhole collapsed region near the splicing point and the free end of the PCF is infiltrated with Agarose.
At the last step, only those peptides which contained at least 3 amino acids at each side of the fusion or splicing point were considered to be able to represent fusion or splicing events and were kept for further analysis.
The pump coupling losses including those at the pump combiner and splice points amount to about 25%.
The reflectance spectra obtained from these two foreoptics were highly correlated (r > 0.95 for most wavelengths) and the 1st derivatives of the spectra were well correlated for all but the detector splice points (r > 0.9 for most wavelengths).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com