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Affinity purification of recombinant proteins has been facilitated by fusion to a modified protein splicing element (intein).
In this report, we used an "intein" fusion system to express Cre as a C-terminal fusion to a modified protein splicing element, i.e., intein.
This system utilizes the inducible self-cleavage activity of a protein splicing element (termed intein) to separate the target protein from the affinity intein CBD tag without using any proteases.
The conformational switch places each splicing element into different structural contexts.
ASSEDA calculates R i,total by adding the R i value of a regulatory splicing element to the contributions of constitutive splice sites, and applying a second gap surprisal term based on the frequency of distance from the splicing element to the nearest natural site.
Once the dysfunctional HEG is fixed in the population (through drift or by the selective disadvantage conveyed by the active HEase), the splicing element can be deleted, thus returning the homing cycle to its beginning.
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Though he was not the first modern dancer to study ballet, his way of splicing elements from both genres in his own work was a breakthrough.
In the past decade, mechanistic studies and extensive engineering of the naturally occurring protein splicing elements, termed inteins, has led to the development of numerous novel technologies.
Note that several splicing elements, such as the 5′ splice site, 3′ splice site, branch site and polypyrimidine tract, are needed in almost all kinds of splicing processes (Black, 2003), it is thus expected that there must be other motifs contribute to tissue-specific manner, and the preference of some motifs should be different in different tissues.
Since AS often show tissue-specific manner, and that the abundances of splicing factors are not likely expressed equally across different tissues (Grosso et al., 2008), one may wonder how cis- splicing elements co-operate with these splicing factors within tissues.
Strict selection control of exonic splicing elements explains the rarity of SNPs near intron-exon boundaries [102].
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