Screening of a larval CNS cDNA library revealed three putative receptor subtypes and nucleotide sequence comparisons suggest that they are produced through alternative splicing at the 3′-end.
Recent developments in sequencing technologies and computational biology have allowed researchers to investigate alternative splicing at an unprecedented scale and resolution.
Moreover, we demonstrate the autonomy of both the EDB and the IIICS alternatively spliced regions in adult mice lacking regulation of the alternative splicing at the EDA exon.
Next-generation sequencing technologies, with reduced time and cost, provide unprecedented opportunities for deep interrogation of alternative splicing at the genome-wide scale.
However, our understanding of alternative splicing at the genomic level remains limited.
Unexpectedly, we identified 2 new events of alternative splicing at both extremities of IKBKAP coding sequence (Figure 6A).
Most polymorphisms in a GT-AG sequence should result in no splicing at that site for one allele.
These can either enhance (exonic splicing enhancers or ESEs) or reduce (exonic splicing silencers or ESSs) splicing at nearby splice sites.
In order to determine if the drug causes activation of IRE1α signaling, we measured XBP1 mRNA splicing at different times after the METH injection.
We did not observe splicing at canonical splice sites in our in vitro system, and most events occurred between short direct repeats.
This is an important application, but the sequence-level detail of RNA-Seq data provides the power to examine alternative splicing at a finer level of detail.
