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Several mycobacteriophage inteins, sequences able to splice out of a protein precursor, have been described previously, one of which has been shown to have novel biochemical activities [41].
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Additionally, an alternatively spliced, out-of-frame fusion transcript was detected.
A straightforward prediction suggests that in T Wis transcripts, splicing "out of" exon 7 would simply fail and result in a transcript terminating with the ETn element.
Many class I and II RNR genes are interrupted by introns and/or inteins which are spliced out for sequencing of RNA or protein.
These may splice out some of these domains to produce other membrane bound versions of the protein or secreted forms.
On the other hand, RBPs are essential for mRNA maturation, which involves the addition of a 7-methylguanosine cap at the 5'end of mRNA-precursors, the splicing-out of introns, editing, and the addition of a polyadenosine tail at the 3'end of the message.
The successful spread and retention of mobile introns depends on expression of the mobility-promoting protein from within the intron, and on accurate splicing-out of the introns from the flanking exon sequences.
In females, the male-specific exons are spliced out because of the presence of Tra protein [ 16- 18].
We found that, on average, exons that are spliced out in part of the mRNA have significantly weaker acceptor sites.
Exon-19 was spliced out in six of the cDNA clones sequenced (Cl.44-43 and 38 and 39).
Thus, to generate switchgrass monofunctional LKR transcripts, instead of splicing out intron 12 at least part of the intron is retained and poly-A added.
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