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Exact(4)
Flag (the gene encoding the capture spiral filament) from Nephila clavipes [41] and MaSp2 from Argiope trifasciata [38] both show highly repetitive exon-intron gene organizations in which sequential introns within a gene have nearly identical nucleotide sequences.
These proteins are constituents of three spider silk fiber types: the dragline silk composed of MaSp1 and MaSp2, the capture spiral filament of flagelliform silk protein (Flag), and the eggcase fibers produced from tubuliform (also called cylindrical) gland proteins (TuSp1, CySp1 and CySp2).
The AFM and EM analysis suggested a structure of the Snf7 assemblies, where a single spiral filament interacted laterally with itself to form double-stranded filaments.
Since Snf7 filaments can curl into circles or spirals (Hanson et al., 2008, Henne et al., 2012, Shen et al., 2014), we reasoned that one patch could be made of a single spiral filament growing from its tips at constant rate.
Similar(56)
Three-dimensional electron tomography of the midbody revealed 17-nm-thick spiral filaments adjacent to membrane constrictions, analogous to spirals of CHMP4B at the plasma membrane that were observed by deep-etch EM.
The severe limitations of immunolabeling in electron tomography made it impossible to confirm that CHMP4B (or other ESCRT-III subunits) comprise the 17-nm spiral filaments in the midbody, although this identity seems likely, given that formation of these filaments was dependent upon expression of a different subunit of the ESCRT-III complex.
Handles were spiraling filaments, feet shaped like teardrops.
Not clear was whether (and how) rings might convert into spiraling filaments.
Previous studies demonstrated that a particular ESCRT complex, called ESCRT-III, forms spiraling filaments on membranes to generate vesicles.
Strikingly, membranes from cells co-expressing 'open' CHMP4A α1 α5) and full-length CHMP2A were studded with eversions lined by spiraling filaments.
The study makes four important claims: 1) Conical spiraling filaments of ESCRT-III proteins can be observed at the plasma membranes of cells lacking Vps4 proteins.
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