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The experimental and numerical simulation results show that the suggested approach reduces 97% thermal displacement compared with the motorized spindle without the suggested thermal error control system.
But to achieve the desire hardness and strength it is difficult to get the spindle without bending in heat treatment due to the length of spindle is too high to its diameter.
These results suggest that the MK2-deficient oocytes contain a collapsed spindle without spindle poles.
Paternal pronuclei underwent NEBD prior to pronuclear meeting and formed the spindle without maternal chromosomes.
In all trypanosomatids, the nuclear chromosomal segregation takes place during a closed mitosis, with formation of an intranuclear spindle without disruption of the nuclear envelope [25].
We observed that, in WT zygotes, the compartment stained by LCA-FITC formed a granular layer around the mitotic spindle without invading the structure.
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However, many systems assemble anastral spindles, i.e., spindles without centrosomes at the poles.
We further used monastrol, which arrest the cells in mitosis with mono-astral spindles without interfering with microtubule dynamics [31].
Differently from 4-HPR and other retinoids, 4-oxo-4-HPR targets microtubules and inhibits tubulin polymerization causing mitotic arrest and formation of multipolar spindles without loss of centrosome integrity [13].
To test how kinetochore-fiber dynamics modulate spindle shape, we generated long spindles without disrupting kinetochore-fibers by depleting the microtubule depolymerase Kif18a.
In contrast, MLN8237-treated samplexhibitedted cells with non-bipolar or multi-polar spindles without detection of p-AURKA (Fig 1D lower), indicating that MLN8237 inhibited phosphorylation of AURKA, impaired the formation of the bipolar spindle, and blocked mitosis.
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