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This cytoplasmic area occupied by the EMTs at PPB maturation is still maintained at the spindle stage.
During the spindle stage, the perpendicularity of the spindle to the cell division plane is often lost because of its rotation.
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(C ) Addition of 1 µM recombinant importin α to steady-state spindles in stage 8 extracts decreases kif2a recruitment to spindles in addition to increasing spindle size.
Compared with other methods, an MBAS is less susceptible to spindle error (stage-independence) when detecting angles, can maintain high sensitivity with miniaturized size, and can be used conveniently at the factory level.
We recorded 306 channels of MEG simultaneously with 60 channels of EEG during naturally occurring spindles of stage 2 sleep in 7 healthy subjects.
(C ) Inhibition of the RanGTP gradient by addition of 1 µM RanT24N disrupts steady-state spindles in stage 3 but not stage 8 extracts.
Changes in kif2a spindle recruitment between stage 3 and stage 8 spindles occurred without significant changes in protein levels.
10.7554/eLife.00290.019 Figure 7. Metaphase spindle orientation in stage 3 embryos depends on spindle size.
Stage 3 spindles were similarly disrupted by this treatment, resulting in a significant (p<0.001, comparing treated to control) loss of tubulin intensity in spindle center, while stage 8 extract spindles were unaffected under the same conditions (p>0.01, Figure 2C,D).
(B ) Quantification of spindle orientation in stage 3 embryos after microinjection of kif2a or kif2a ΔNLS at metaphase.
(A ) Quantification of spindle orientation in stage 3 embryos after microinjection of kif2a or kif2a ΔNLS at prophase and anaphase.
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