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Surface finish and shape of a machined part rely directly on the overall accuracy in motion of the tooling spindle over the entire machining parameter and speed range.
(A ) Time-lapse sequence showing a single cell with a bipolar spindle over the course of 129 s.
To monitor the relative position of the metaphase plate in the spindle over time, we recorded by time-lapse imaging HeLa cells stably expressing eGFP-centrin1 (centriole marker) and eGFP-CENPA (kinetochore marker) and automatically tracked centrosomes and the metaphase plate using an in-house developed software (Jaqaman et al., 2010; Vladimirou et al., 2013).
On some machines, it's done by pushing the bobbin spindle over to one side.
However, when set up correctly this arrangement supports the spindle over a much greater length and provides totally smooth and vibration-free running.
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"If it's such a big hit, then let's see what we can hit with it!" At that, my father yanks the small vinyl record off the spindle, walks over to our bedroom window and sets sail.
Several studies have recorded sleep spindles over lateral and medial prefrontal [45], [46], medial temporal [46], [47], and parietal cortices [48].
The distribution of fast and slow spindles has been reported to be bimodal (Astill et al., 2014) and corresponds to an approximate topographical localization, fast spindles having a predominant distribution over parietal regions and slow spindles over prefrontal regions (Schabus et al., 2007; Andrillon et al., 2011).
In order to monitor spindle changes over a period of time in actual living cells, a chromosomally integrated GFP-tagged α2-tubulin gene [17] was introduced into dis3-54.
Support for this proposal also comes from the report that the caspase-dependent degradation of the kleisin subunit of condensin I in human cells, arrested by spindle poisons over long periods of time, results in an increase in chromosome width [ 97].
You need to ensure the spindle will not over-extend outward.
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