First, we focus on the problem of spindle length determination.
McNally, K., Audhya, A., Oegema, K. & McNally, F. J. Katanin controls mitotic and meiotic spindle length.
In vivo studies have implicated kinesin-14 in spindle length control1,5 and spindle pole focusing2,3.
Although a number of molecular perturbations have been shown to influence spindle length, a global understanding of the factors that determine metaphase spindle length has not been achieved.
We then discuss models for force integration and spindle length determination.
Phosphorylation decreases severing activity in Xenopus egg extracts and is involved in controlling spindle length.
Cai, S., Weaver, L. N., Ems-McClung, S. C. & Walczak, C. E. Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules.
Cai, S., Weaver, L.N., Ems-McClung, S.C. & Walczak, C.E. Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules.
Hagan, I. M., Riddle, P. N. & Hyams, J. S. Intramitotic controls in the fission yeast Schizosaccharomyces pombe: the effect of cell size on spindle length and the timing of mitotic events.
Kinesin-14's role in spindle length control might be to mediate microtubule nucleation26 rather than exerting direct pushing or pulling forces against other crosslinking motors such as kinesin-56,27−29.
The kinesin-13 KLP10A motor regulates oocyte spindle length and affects EB1 binding without altering microtubule growth rates.
