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The regulation of metazoan spindle elongation is more complex.
Rapid inhibition of Plk1 blocked spindle elongation and cytokinesis.
Chromosomes are segregated by chromosome to pole movement and spindle elongation in anaphase.
Our results imply that spindle elongation and cytokinesis are separable events that are independently controlled.
Instead, Plk1 activity at anaphase onset is necessary for spindle elongation to occur.
Timelapse data was analyzed to calculate contractile ring ingression and spindle elongation.
Removal of all chromosomes from the spindle does not result in anaphase spindle elongation [7] thus there must exist a trigger other than chromosome cohesion to initiate spindle elongation.
Quantitation of the anaphase B defects in PtK2 cells (Figure 2B) and HeLa cells (Figure 2C) showed that no spindle elongation occurred during the time period when spindle elongation completed in control cells.
Plk1 inhibition did not affect anaphase A chromosome to pole movement, but blocked anaphase B spindle elongation.
Thus Plk1 is a candidate for controlling anaphase spindle elongation but its role in the process has not been defined.
Their down-regulation led to dramatic defects in chromosome congression/segregation frequently accompanied by mitotic spindle elongation.
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