Recent work has shown an intricate relationship between the Theileria schizont and host cell mitotic and central spindles, dependent on recruitment of host cell Polo-like kinase (von Schubert et al., 2010).
However, the servo theory at the time conceived a muscle spindle-dependent reflex mechanism automatically subserving motor control without conscious intervention.
AURORA A is a PCM protein, and its targeting to and subsequent activation at the mitotic spindle are dependent on another centrosomal protein CEP192 (centrosomal protein of 192 kDa) (discussed later) [ 61].
Sas-6 siRNA: one possibility is that Sas-6 siRNA could affect the position of the metaphase plate, microtubule stability and SKAP loading on kinetochores and/or lead to a spindle checkpoint dependent anaphase delay as an off-target effect.
Spindle microtubule-dependent deformation of both inner kinetochores and the HEC1/Ndc80 microtubule-capturing module, then results in kinetochore separation from the Aurora B pool and ensuing reduced kinase activity at centromeres.
Interestingly, Plk1/Mad2-depleted cells efficiently degraded GFP-Cyclin B1-NT (Fig. 1A), with kinetics very similar to Monastrol-treated control cells (Fig. 1) confirming that Plk1 is not required for activation of spindle checkpoint-dependent APC/C-Cdc20 activity.
Previous studies addressing the activation of the spindle checkpoint-dependent APC/C-Cdc20, required for the degradation of Cyclin B1 and Securin, have also failed to show a requirement for Plk1 [37], [38], [38].
Lte1 interacts with Tem1 [ 22], and while it is tempting to speculate that it thus activates MEN in a spindle elongation-dependent manner, this remains to be demonstrated.
Following pre-synchronization with thymidine, cells were released and arrested in mitosis by the addition of either nocodazole or the Plk1-inhibitor TAL (Santamaria et al. 2007), both of which cause a spindle checkpoint-dependent prometaphase arrest.
The two-way ANOVA for fast F3 spindles and dependent measure alpha SRS/SRD power with repeated measures factor TIME and between-subject factor ENHANCEMENT revealed a significant main effect for factor ENHANCEMENT (F 1,14) = 8.606, p = 0.011) indicating more alpha power during fast spindles (0 1200 ms) for subjects who improved overnight (Fig. 3A).
