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Kinesin spindle protein (KSP) is a mitotic kinesin that is expressed only in proliferating cells and plays a key role in spindle pole separation, formation of a bipolar mitotic spindle, as well as centrosome separation and maturation.
During prometaphase and metaphase, C13orf3 localised to the mitotic spindle as well as to the kinetochores.
As with the yeast SCD census, we found an unexpected abundance of proteins belonging to the microtubule organizing center and spindle as well as the actin cytoskeleton.
The amplitude and the position of these models yield the signals and position of the photoactivated spindle as well as the signal intensity background spindle.
Here, the deconvolution operation identified artificial clusters of green fluorescence as the underlying distribution of fluorescent kinetochores, both in the spindle as well as outside of the spindle (Fig. 1d).
This chromosomal pool of dynein was difficult to distinguish on prometaphase and metaphase bipolar spindles, potentially due to the fact that dynein localized all over the spindle as well, which could obscure a faint chromosomal pool.
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We show that while both neural approaches increase the control precision and robustness, the DGFNN controller gives the best results for reducing the speed deviation and suppressing the torsional vibration of the spindles, as well as is more computationally efficient.
Monoastral, anastral, and multipolar spindles, as well as unfocused broad-based spindles were documented.
In our experiments, ERK3 was detected on the fibers of the abnormal spindles as well as cytoplasmic asters.
Additionally, Wang et al. demonstrated that Plk3 localizes to the spindle poles and mitotic spindles, as well as to the midbody during metaphase and telophase, respectively [9].
It seems likely that most of proteins physically present on microtubules of meiotic spindles will be found on mitotic spindles as well.
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