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Higher magnification image of the mouse spinal section shown in white-dashed box in Fig. 6h, stained with Map2 for mouse neuronal cell bodies and dendrites (green).
Spinal section were then incubated with a mixture of Alexa Fluor 488-labeled chicken anti-mouse IgG antibody (1 400, cat. A-21200; Molecular Probes, Eugene, OR, USA; green fluorescence) and DyLight 549-conjugated donkey anti-rabbit IgG antibody (1 400, cat. 711-506-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA; red fluorescence) for 40 min at room temperature.
All animals, except three, had a correct spinal section.
In the spinal section provided in Figure 2A, the gray matter has been outlined together with its classical subdivisions in Rexed laminae (I-III; IV-VI, VII, VIII, IX, and X) [39].
Unfortunately few measures for this spinal section are reported in the literature.
At sampling, fish were transferred to an anaesthetic/sedative solution (ethyl 4-aminobenzoate; 0.1 g/l; Sigma-Aldrich) and killed by spinal section.
Similar(53)
Dmrt3+ interneurons in P11 spinal sections were positive for Viaat (also known as Slc32a1), marking inhibitory neurons, but negative for Vglut2 (also known as Slc17a6), marking the majority of spinal excitatory neurons16 (Fig. 3f).
Spinal sections were then incubated with DyLight 549-conjugated anti-rabbit IgG antibody (1 400) for 40 min at room temperature.
Spinal sections were then incubated with a mixture of Alexa Fluor 488-labeled chicken anti-mouse IgG antibody (1 400) and DyLight 549-conjugated donkey anti-rabbit IgG antibody (1 400) for 40 min at room temperature.
Quantification was performed in spinal sections obtained from the transection site.
Transverse frozen spinal sections (30 µm thickness) were cut in a cryostat, collected serially into two dishes.
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