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For the 5+ DP 32 polymer, observed only in 150 °C ESI and SPIN sources, the detected ion intensity for SPIN is over an order of magnitude higher than what was detected in the 150 °C ESI configuration.
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For this project you'll need eight bananas, a bunch of alligator jumper wires, a Quickstart board, a 220 ohm resistor, and the spin source archive.
Many analytes were also detected with higher charge states using the SPIN source.
The combination of the lactone bond stabilization and gentler ionization conditions present in the SPIN source aided the MS analysis.
The SPIN source increases both signal intensities and charge states and aids in the detection of labile sialylated glycans by providing gentler ionization conditions at the MS interface.
The SPIN source facilitated sialic acid detection by providing enhanced signal intensities, higher charge states, and apparently, gentler ionization and ion transfer.
In the SPIN source configuration the ESI emitter was moved to the first vacuum region of the mass spectrometer and positioned at the entrance of the ion funnel.
Lactone profiles for various DPs observed in multiple charge states from the SPIN source configuration are further shown in Figure 5a.
Here we present evidence that the SPIN source not only improves MS sensitivity but also facilitates the detection of more labile compounds.
The SPIN source also demonstrated a shift toward higher charge states allowing for extended coverage by reducing the m/ z of the detected polymers.
The SPIN source enabled the detection of colominic acid compounds with higher DPs and intact sialylated glycans with significantly reduced in-source fragmentation without any sacrifice in MS sensitivity.
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